| 质粒名称: | pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32149 |
| 存储实验室: | Gerald Rubin |
|
载体骨架基本信息 |
|
| 载体名称: | pJFRC2-10XUAS-IVS-mCD8::GFP |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 昆虫细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FRT> STOP STOP FRT> |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Bgl2(未破坏) |
| 3' 克隆位点: | XhoI (未破坏) |
| 5' 测序引物 | hsp70 F: GAGCGCCGGAGTATAAATAGAG |
|
文献引用方法 |
|
| 材料和方法部分: | pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP was a gift from Gerald Rubin (爱迪基因 plasmid # 32149) |
| 参考文献部分: | Multiple new site-specific recombinases for use in manipulating animal genomes. Nern A, Pfeiffer BD, Svoboda K, Rubin GM. Proc Natl Acad Sci U S A. 2011 Aug 9. 10.1073/pnas.1111704108 PubMed 21831835 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP 9951 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9951)
AUTHORS Nern A, Pfeiffer BD, Svoboda K, Rubin GM
TITLE Multiple new site-specific recombinases for use in manipulating
animal genomes.
JOURNAL Proc Natl Acad Sci U S A. 2011 Aug 9.
PUBMED 21831835
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP|
FEATURES Location/Qualifiers
source 1..9951
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 31..135
/gene="bla"
/label=AmpR promoter
CDS 136..996
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(354..373)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1167..1755
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1656..1675
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
protein_bind 1842..1911
/label=attB
/bound_moiety="phage phi-C31 integrase"
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
primer_bind complement(2136..2154)
/label=pCasper-F
/note="Drosophila mini-white gene, reverse primer"
CDS join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
4864..5478)
/codon_start=1
/gene="white"
/product="Drosophila white gene eye color pigment"
/label=mini-white
/note="This is a modified version of the white gene lacking
part of the first intron."
/translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
protein_bind 6508..6602
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
protein_bind 6636..6730
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 6761..6999
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind 6946..6967
/label=pCasper-hs
/note="Drosophila Hsp70 promoter, forward primer"
protein_bind complement(7009..7042)
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
intron 7487..7552
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 7682..7702
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 7974..8108
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind complement(8126..8159)
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
intron 9234..9299
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9429..9449
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9721..9855
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9897..9915)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
6721 gtcctccgag cggagactct agcgctagcg acgtcgagcg ccggagtata aatagaggcg
6781 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
6841 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
6901 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
6961 tgccaagaag taattattga atacaagaag agaactctga atagatctga agttcctata
7021 ctttctagag aataggaact tctaacgtaa gctagctaga ccggtgtcga ctaaagccaa
7081 atagaaaatt attcagttcc tggcttaagt ttttaaaagt gatattattt atttggttgt
7141 aaccaaccaa aagaatgtaa ataactaata cataattatg ttagttttaa gttagcaaca
7201 aattgatttt agctatatta gctacttggt taataaatag aatatattta tttaaagata
7261 attgcgtttt tattgtcagg gagtgagttt gcttaaaaac tcgtttaggt ttgtcctccc
7321 gaaattattt atttaaatgc gatggagagt tggcgccgaa tcgaaaactt tacgcgctta
7381 aaagcacgag ttggcatccc taacgcgtag gatctttgtg aaggaacctt acttctgtgg
7441 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
7501 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
7561 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
7621 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
7681 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
7741 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
7801 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt
7861 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
7921 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
7981 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
8041 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
8101 tctggatcac tagtgatctg gccgggaagt tcctatactt tctagagaat aggaacttcc
8161 tcgagaatca aaatgggcaa caaatgctgc agcaagcgac aggatcagga actggcactg
8221 gcctatccca ctgggggcta caagaaatcc gactacacct ttggccagac gcacatcaac
8281 agcagcggcg gcggcaacat gggcggcgtt cttggccaga agcataacaa cggtggctcg
8341 ctggactcgc gctacacgcc cgatcccaat catcggggtc cgttgaaaat cggcggaaag
8401 ggcggcgttg acatcatcag accacgcgga tccatgtcca aaggtgaaga actgtttacc
8461 ggagtagtcc cgatattggt tgaactcgac ggcgatgtca acggtcataa attcagtgtg
8521 tccggcgagg gtgagggcga cgccacatac ggtaagctga cgttgaagtt catatgcacc
8581 acgggcaagc tgcccgtgcc atggccgacg ttggtcacga cgctgacgta tggtgtccag
8641 tgtttcagcc gttaccccga tcatatgaag cagcatgact ttttcaagtc ggcgatgccg
8701 gagggatacg ttcaagagag gaccattttc ttcaaggatg acggcaacta taagacgcga
8761 gcggaggtga aatttgaagg cgacacactc gttaaccgta ttgagttgaa gggcattgat
8821 tttaaggagg atggcaacat tctgggccat aagttggaat acaactacaa ctcgcataat
8881 gtgtatataa tggcagataa gcagaaaaac ggaataaagg ttaacttcaa gattcgccac
8941 aacatagagg acggttccgt gcaacttgca gatcattacc aacagaacac acccattgga
9001 gatggcccag ttctcttgcc agacaatcac tacctttcca cacagtccgc gttgagcaag
9061 gaccccaatg aaaagcggga ccacatggtg ttgctggagt ttgtgaccgc agctggtatt
9121 acacacggca tggatgagct ctacaagtaa tctagaggat ctttgtgaag gaaccttact
9181 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
9241 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
9301 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
9361 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
9421 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
9481 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
9541 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat ttgatgtata
9601 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
9661 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
9721 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
9781 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
9841 tatcatgtct ggatcgatct ggccggccgt ttaaacgaat tcttgaagac gaaagggcct
9901 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt a
//
